Development, characterization and validation of an analytical method for solid lipid nanoparticles containing ascorbyl palmitate

Ana Paula Tasquetto da Silva, Marta Palma Alves, Luiz Rodrigues Junior

Resumo


The ascorbyl palmitate (AP) has wide application as an antioxidant additive in pharmaceutical, medical and cosmetic products. However, it is easily degraded when used as a free molecule. The nanoencapsulation has been used to overcome the degradation of several unstable molecules. In this work, solid lipid nanoparticles (SLN) containing ascorbyl palmitate were developed and characterized, and an analytical method of validation was proposed for their quantification. The results demonstrated that the SLN-containing AP had an initial particle size of 86.41 ± 0.30 nm and 89.27 ± 0.86 nm after 90 days; the values of polydispersity index were around 0.2; the initial zeta potential was -12.20 ± 0.23 mV and -18.40 ± 0.29 mV after 90 days; the pH was approximately 4.0. The method showed linearity between 9-24 µg.ml, and correlation coefficient represented by r = 0.9998. The analysis of precision and accuracy showed a low relative standard deviation (<2.98%) and a sufficient recovery percentage of AP (101.40%). The procedure provided specificity, linearity, precision, accuracy and robustness, indicating that the method can be applied to the quantitation of AP at SLN.

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DOI: https://doi.org/10.37779/nt.v17i2.1886

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